dsGreen Gel Staining Solution, 10000×
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產品名稱: dsGreen Gel Staining Solution, 10000×
產品型號: A0010 10010 20010
產品展商: 其它品牌
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簡單介紹
dsGreen Gel Staining Solution, 10000×
A0010 10010 20010
dsGreen Gel Staining Solution, 10000×
的詳細介紹
dsGreen Gel Staining Solution, 10000×
PCR and qPCR
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Cat. #
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Quantity
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Price
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Lead time
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Buy this product
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A0010
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50 uL
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29.00$
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in stock
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10010
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0.5 mL
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100.00$
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in stock
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20010
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1 mL
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180.00$
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in stock
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Bulk inquiry 
dsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.
Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.
Comparison between ethidium bromide and dsGreen
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Feature
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Ethidium Bromide
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dsGreen
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Fluorescence
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Red (615 nm)
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Green (524 nm)
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Excitation maximum
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302 nm
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454 nm
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Excitation light source
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UV only
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Blue light or UV
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Sensitivity
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2 ng / band (dsDNA)
100 ng / band (RNA)
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0.08 ng / band (dsDNA)
1–2 ng / band (oligonucleotides)
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Health hazard
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High
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Low
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Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
Download in
ChemDraw format
Recommended protocol
DNA Staining in Gels with dsGreen or SYBR Green I
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General properties
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Appearance:
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orange solution
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Quality control:
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NMR 1H, HPLC-MS (95%), functional testing
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Storage conditions:
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Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
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MSDS:
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Download
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Spectral properties
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Excitation maximum, nm:
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454
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ε, L?mol?1?cm?1:
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73000
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Emission maximum, nm:
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524
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Fluorescence quantum yield:
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0.8
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Product citations
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Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, 2017, 220(2), 251–262. doi: 10.1111/apha.12820
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Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology, 2017, 232(6), 1559–1570. doi:10.1002/jcp.25682
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Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. Evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology, 2016, 7, 773. doi:10.3389/fmicb.2016.00773
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Jain, D.; Siede, W. Rad5 Template Switch Pathway of DNA Damage Tolerance Determines Synergism between Cisplatin and NSC109268 in Saccharomyces cerevisiae. PLoS ONE, 2013, 8(10), e77666. doi: 10.1371/journal.pone.0077666
