dsGreen for Real-Time PCR, 100×
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產品名稱: dsGreen for Real-Time PCR, 100×
產品型號: 41010
產品展商: 其它品牌
產品文檔: 無相關文檔
簡單介紹
dsGreen for Real-Time PCR, 100×
41010
dsGreen for Real-Time PCR, 100×
的詳細介紹
dsGreen for Real-Time PCR, 100×
PCR and qPCR
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Cat. #
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Quantity
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Price
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Lead time
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Buy this product
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41010
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1 mL
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310.00$
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in stock
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Bulk inquiry 
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
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Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
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PCR tested preparation – quality guaranteed
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Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
Download in
ChemDraw format
qPCR curves with dsGreen
Recommended protocol
qPCR with dsGreen or SYBR Green I
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General properties
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Appearance:
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light orange solution
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Quality control:
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NMR 1H, HPLC-MS (95%), PCR testing
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Storage conditions:
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Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
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MSDS:
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Download
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Spectral properties
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Excitation maximum, nm:
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454
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ε, L?mol?1?cm?1:
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73000
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Emission maximum, nm:
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524
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Fluorescence quantum yield:
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0,8
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Product citations
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Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038
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Revalde, J.L.; Li, Y.; Wijeratne, T.S.; Bugde, P.; Hawkins, B.C.; Rosengren, R.J.; Paxton, J.W. Curcumin and its cyclohexanone analogue inhibited human Equilibrative nucleoside transporter 1 (ENT1) in pancreatic cancer cells. European Journal of Pharmacology, 2017, 803, 167–173. doi: 10.1016/j.ejphar.2017.03.055
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Wu, Z.-G.; Jiang, W.; Chen, S.-L.; Mantri, N.; Tao, Z.-M.; Jiang, C.-X. Insights from the Cold Transcriptome and Metabolome ofDendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation. Frontiers in Plant Science, 2016, 7, 1653. doi: 10.3389/fpls.2016.01653
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Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J.Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community.Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795
SYBR® is a registered trademark of Molecular Probes.
