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Abbexa
TMEM232 siRNA
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產品名稱:
TMEM232 siRNA
產品型號:
abx937276
產品展商:
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產品文檔:
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簡單介紹
TMEM232 siRNA
TMEM232 siRNA
的詳細介紹
siRNA to inhibit TMEM232 expression using RNA interference.
This product is provided as two 5 nmol vials (10 nmol), three 5 nmol vials (15 nmol) or 2x three 5 nmol vials (30 nmol) of lyophilized siRNA oligo duplexes. Each vial contains slightly different sequences to ensure full knockout of the gene. Each vial contains slightly different sequences to ensure full knockout of the gene. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. The siRNA oligos are also chemically modified (2'-OMe) for increased stability and enhanced knockdown in vitro and in vivo.
Target
TMEM232
Reactivity
Rat
Host
Synthetic
Tested Applications
RNAi
Purity
> 97%
Form
Lyophilized
Specificity
TMEM232 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Storage
Shipped at 4 °C. Store at -20 °C for up to one year.
Swiss Prot
B1WBT0
GeneID
501199
Gene Symbol
TMEM232
Directions for use
1. Transfect with 100 nM siRNA 48 to 72 hours prior to cell lysis.
2. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.
3. Resuspend the siRNA oligos to an appropriate concentration with DEPC water.
Each vial is suitable for 250 transfections in a 24 well plate (20 pmol for each well).
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Availability
Shipped within 5-10 working days.
Note
This product is for research use only.
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