Cardiolipin IgG EULISA (212796)
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產(chǎn)品名稱: Cardiolipin IgG EULISA (212796)
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Cardiolipin IgG EULISA (212796)
Cardiolipin IgG EULISA (212796)
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Cardiolipin IgG EULISA
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The EULISA Cardiolipin IgG is a fast and reliable ELISA test kit for In Vitro diagnosis of patients with anti-phospholipid syndrome (APS), an autoimmune disorder characterised by thrombosis in both arteries and veins.
Tests
Break apart micro titration strips (12x8) 96 wells
Calculation
Qualitative and quantitative
Incubation time
30+30+30 min
Detection system
HRP/450 nm
Availability
CE marked. Not for sale in US
- Intended use
- Product Details
- Kit Components
Intended use
Enzyme immunoassay for the detection of autoantibodies IgG against Cardiolipin (CL).
Background
The anti-phospholipid syndrome (APS) is an autoimmune disorder which can comprise clinical conditions as venous and arterial thrombosis, thrombocytopenia, myocardial infarction, recurrent spontaneous abortion and neurological complications.
Cardiolipin (CL) is the most common, negatively charged, acid phospholipid. Autoantibodies correlated with APS are directed not only against CL and similar phospholipides but also against phospholipid/protein complexes. ?2-glycoprotein 1 (?2-GP-1; = apolipoprotein H) has been identified as such a natural and essential co-antigen for CL-autoantibodies. The occurrence of CL/?2-GP-1 autoantibodies is associated with a tendency towards thrombosis. Besides this diagnostic significance, these antibodies are believed to be directly involved in the pathogenesis of APS.
Technical information
The EULISA Cardiolipin IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against CL/?2-GP-1. The immobilised antigen is a combination of CL, isolated from bovine heart, and a highly purified preparation of native ?2-GP-1. The test is fast (incubation time 30 - 30 - 30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.
Kit components and storage of reagents
Contents of the kit
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1 microwell plate, coated with CL/?2-GP-1 and hermetically packed in a foil laminate pouch together with a desiccant bag.
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Sample buffer, 100 mL, ready-to-use, orange coloured.
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Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
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6 calibrators, 2.0 mL each, 0, 3.0, 8.0, 18, 45 and 100 GPL-U CL/?2-GP-1 antibodies (IgG)/mL, ready-to-use, gradually blue coloured.
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Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
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Anti-human IgG HRP conjugate, 14 mL, ready-to-use, red coloured.
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Substrate solution, 14 mL, ready-to-use, colourless.
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Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
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Directions for use
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Lot-specific certificate of analysis
All kit components are stored at 2 - 8°C.
