JIB-04(NSC693627),JumonjiHistoneDemethylaseInhibitor
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產(chǎn)品名稱: JIB-04(NSC693627),JumonjiHistoneDemethylaseInhibitor
產(chǎn)品型號: M60132-2s
產(chǎn)品展商: 其它品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
JIB-04(NSC693627),JumonjiHistoneDemethylaseInhibitor
JIB-04(NSC693627),JumonjiHistoneDemethylaseInhibitor
的詳細(xì)介紹
Product Information
| Molecular Weight: |
308.76 |
| Formula: |
C17H13ClN4 |
| Purity: |
≥98% |
| CAS#: |
n/a |
| Solubility: |
DMSO up to 50 mM |
| Chemical Name: |
(E)-5-chloro-2-(2-(phenyl(pyridin-2-yl)methylene)hydrazinyl)pyridine |
| Storage: |
Powder: 4oC 1 year.
DMSO: 4oC 3 month;
-20oC 1 year. |
Biological Activity:
JIB-04 (NSC693627, E-isomer) is a potent, selective and cell permeable Jumonji histone demethylase inhibitor. Unlike the other known inhibitors, JIB-04 is not a competitive inhibitor of a-ketoglutarate. It inhibits the demethylase activity of Jumonji enzymes in vitro, with IC50?~230 nM for ?JARID1A (KDM5A), ~440 nM for JMJD2A (KDM4A) and JMJD2B (KDM4B), ~340 nM for JMJD2E (KDM2E), and ~1 μM for JMJD3 (KDM6B) and JMJD2C (KDM4C). JIB-04 blocks Jumonji demethylase activity in cells and consequently inhibits cell growth, without affecting other a-ketoglutarate-dependent hydroxylases or histone-modifying enzymes, especially HDACs. JIB-04 alters transcriptional programs in cancer but not in normal cells, leading to cancer-specific cell death. Importantly, in vivo, JIB-04 lowers histone demethylase activity in tumors, reduces tumor burden and prolongs survival of mice in an aggressive breast cancer model.
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How to Use:
- In vitro:??JIB-04 was used at 1 μM final concentration in various in vitro assays.
- In vivo:?JIB-04 was administered 2–3 times weekly by IP injection at 110 mg/kg in sesame oil (H358 xenografts) or by gavage in Cremophor EL at 55 mg/kg (A549 xenografts) for 4-6 weeks.
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Reference:?
- 1. Wang L, et al. A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth. (2013) Nat Commun. 4:2035
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Products are for research use only. Not for human use.
