For Research Use Only. Not for use in diagnostic procedures.

Purified human complement component C3. The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD). The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/mL.

All Quidel complement components are tested for functional activity in a standard lytic or applicable functional assay and for biochemical purity by SDS-polyacrylamide gel electrophoresis. Please refer to the Certificate of Analysis for lot specific information on functional titer.

Quidel's purified human complement components are useful in a wide variety of immunochemical and research applications. For assistance with a specific protocol, please contact Quidel Technical Support.

References

Marks, R., Hindmarsh, E. Complement activation occurs on subendothelial extracellular matrix in vitro and is initiated by retraction or removal of overlying endothelial cells, J Immunol 160:6128-6136, 1998.